荧光相关光谱技术(FCS)监测可降解纳米药物载体在血液循环中的“命运”
发布时间：
2023-05-06
分类：
德国美茵茨大学马克斯·普朗克高分子研究所的Kaloian Koynov团队开发了一种基于荧光相关光谱技术（FCS）在少于50μL的全血样本中精确表征荧光标记的纳米药物载体（NCs）的大小、浓度及载药效率。由于在可见光范围内，血液对光的强烈吸收以及大尺寸血细胞会完全占据FCS检测体积，阻碍荧光信号的检测。因此，在全血样本中应用血浆分离膜作为分离介质，使血液中的液体部分及NCs可通过检测体积，同时阻止较大的血细胞进入，从而实现了在血液中监测NCs的“命运”。本文首先采集了Alexa 647与NCs在血浆、全血（过血浆分离膜）及PBS中的FCS数据，然后将NCs注射到小鼠体内，每隔特定时间进行采样。结果显示：在常规血浆和全血中检测到的自相关曲线基本一致，表明新开发的血滴法FCS可成功用于监测和表征血液中的NCs。应用以上方法将为监测NCs在血液循环中的完整性提供新的思路，从而有助于开发具有瞬时稳定性和可预测生物降解性的NCs。
Abstract
The use of nanoparticles as carriers to deliver pharmacologically active compounds to specific parts of the body via the bloodstream is a promising therapeutic approach for the effective treatment of various diseases. To reach their target sites, nanocarriers (NCs) need to circulate in the bloodstream for prolonged periods without aggregation, degradation, or cargo loss. However, it is very difficult to identify and monitor small-sized NCs and their cargo in the dense and highly complex blood environment. Here, we present a new fluorescence correlation spectroscopy-based method that allows the precise characterization of fluorescently labeled NCs in samples of less than 50 μL of whole blood. The NC size, concentration, and loading efficiency can be measured to evaluate circulation times, stability, or premature drug release. We apply the new method to follow the fate of pH-degradable fluorescent cargo-loaded nanogels in the blood of live mice for periods of up to 72 h.
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